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Combining site-directed mutations and reconstitution with a locked chromophore analog converts photoactive yellow protein into a highly fluorescent protein tag

CONVERTION OF PHOTOACTIVE YELLOW PROTEIN INTO A HIGHLY FLUORESCENT PROTEIN TAG BACKGROUND Fluorescent protein tags, such as green fluorescent protein (GFP), exhibit fluorescence when viewed under ultraviolet light. Since these tags make it possible to 'see' metabolic interactions, attaching them to proteins, antibodies, and amino acids allows...

Published: 4/19/2022 | Updated: 2/8/2022 | Inventor(s): Masato Kumauchi, Branden Carr, Wouter Hoff

Keywords(s): Research Tools

Category(s): Technology Classifications > Research Tools